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ABSTRACTA rapid and efficient method for large‐scale purification of natural betulinic acid from Zizyphi Spinosi Semen was described. The procedure involved reflux extraction, preparative separation using low‐pressure reverse‐phase liquid chromatography and purification by crystallization. High purity (over 90%) and recovery of betulinic acid were obtained by preparative low‐pressure reverse‐phase...
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