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Recently, we reported that YghZ from Escherichia coli functions as an efficient l-glyceraldehyde 3-phosphate reductase (Gpr). Here we show that Gpr co-purifies with a b-type heme cofactor. Gpr associates with heme in a 1:1 stoichiometry to form a complex that is characterized by a K d value of 5.8±0.2μM in the absence of NADPH and a K d value of 11±1.3μM in the presence of saturating...
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