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tRNA modifications are crucial for efficient and accurate protein synthesis, and modification defects are frequently associated with disease. Yeast trm7Δ mutants grow poorly due to lack of 2′‐O‐methylated C32 (Cm32) and Gm34 on tRNAPhe, catalyzed by Trm7‐Trm732 and Trm7‐Trm734, respectively, which in turn results in loss of wybutosine at G37. Mutations in human FTSJ1, the likely TRM7 homolog, cause...
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