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We quantified the effect of Mg 2+ on thiamine pyrophosphate (TPP) binding to TPP-dependent thiA riboswitch RNA. The association constant of TPP binding to the riboswitch at 20°C increased from 1.2×10 6 to 50×10 6 M −1 as the Mg 2+ concentration increased from 0 to 1mM. Furthermore, circular dichroic spectra under various conditions showed that 1mM Mg 2+...
The thermodynamic parameters of an antiparallel G-quartet formation of d(G 4 T 4 G 4 ) with 1 mM divalent cation (Mg 2+ , Ca 2+ , Mn 2+ , Co 2+ , and Zn 2+ ) were obtained. The thermodynamic parameters showed that the divalent cation destabilizes the antiparallel G-quartet of d(G 4 T 4 G 4...
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