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Fuculose-1-phosphate aldolase (FucA) is a useful biocatalyst with potential applications in chiral synthesis. In this study, the overall kinetic mechanism of FucA from the archaeon Methanococcus jannaschii was studied. The K m values of dihydroxyacetone phosphate (DHAP) and dl-glyceraldehyde were 0.09 and 0.74mM, respectively. Dead-end inhibition by trimethyl phosphonoacetate and dl-threose...
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