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Escherichia coli UvrD protein is a 3′ to 5′ SF1 DNA helicase involved in methyl-directed mismatch repair and nucleotide excision repair of DNA. Using stopped-flow methods we have examined the kinetic mechanism of translocation of UvrD monomers along single-stranded DNA (ssDNA) in vitro by monitoring the transient kinetics of arrival of protein at the 5′-end of the ssDNA. Arrival at the 5′-end was...
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