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Horse heart cytochrome c is cleaved by thermolysin in 50% aqueous TFE (vv) at neutral pH (25°C, 24 h) at the Gly 56 -Ile 57 peptide bond of the 104-residue chain of the protein. Additional, but anyway minor, fragmentation at the Gly 45 -Phe 46 and Met 80 -Ile 81 peptide bonds is also observed. On the other hand, in buffer...
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