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A technique that is increasingly being used to determine the structure and conformational flexibility of biomacromolecules is Pulsed Electron–Electron Double Resonance (PELDOR or DEER), an Electron Paramagnetic Resonance (EPR) based technique. At X-band frequencies (9.5GHz), PELDOR is capable of precisely measuring distances in the range of 1.5–8nm between paramagnetic centres but the orientation...
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