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Abstract. Degenerate primers corresponding to consensus sequences in the catalytic domains of known fungal adenylate cyclases were used to isolate gene-specific homologs from the Dutch elm disease pathogen Ophiostoma novo-ulmi, the dimorphic human pathogen Candida albicans, and the commercial mushroom Agaricus bisporus. All three fungi gave the expected PCR product of about 390 bp. Computer searches...
Abstract.Ophiostoma ulmi and O. novo-ulmi, both causative agents of Dutch elm disease, can be differentiated by their potential to produce constitutive extracellular laccase. The enzyme has been purified from the culture filtrate to apparent electrophoretic homogeneity and has been partially characterized. The laccase was glycosylated and found to have a molecular mass of 79 kDa or 70 kDa by SDS-PAGE...
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