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The location of a series of lipophilic and lipid-attached BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) membrane probes was analyzed by the quenching of BODIPY fluorescence by a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. When attached to the polar headgroup of PE the BODIPY remained near the polar headgroup in depth. However, when attached at the...
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