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We proposed a cell analysis system without optical filters using a filter-free fluorescence sensor which used wavelength dependent absorption depth in a silicon substrate. Excitation light and fluorescence from fluorescently labeled cells were separated and their light intensities were measured at the same time by applying the proposed parameter. This result shows the filter-free fluorescence sensor...
We developed cavity-sealed single-layer graphene drum resonator by using low-pressure dry transfer technique, without using sacrificial layer etching process. Maximum diameter of 45 μm with gap length of 810 nm was obtained. Raman spectra of suspended graphene was found to be 2D/G ratio of 1.73 and few D peak. A dry transfer technique provides the flat device surface with sealed nanocavity, which...
In this paper, the detection of fluorescence from the solution of a fluorescent dye with a submicromolar concentration by the filter-free fluorescence sensor is reported. The filter-free fluorescence sensor has a photo-gate structure and the intensities of excitation and fluorescent lights can be calculated from the photocurrents for different gate voltages. It makes it possible to measure various...
To detect several kinds of neurotransmitters in brain tissue simultaneously, we developed a pattering technique that harden ultraviolet (UV)-curing resin containing an enzyme to fabricate a enzyme-membrane by using lithography method. In this study, an apyrase-membrane was patterned on a 128×128 pixel ion-image sensor to monitor the distribution and dynamics of adenosine triphosphate (ATP) activity...
We have successfully integrated an amperometric ion sensor array on an Si chip which simultaneously detects potassium ion (K+) and sodium ion (Na+). The sensor array has two kinds of selective electrodes which exhibit high sensitivity for K+ over Na+ (K+-selective electrode) and for Na+ over K+ (Na+-selective electrode), respectively. Ion transfer voltammetry method was adopted for the detection of...
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