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Double electron–electron resonance (DEER) at W-band (95GHz) was applied to measure the distance between a pair of nitroxide and Gd 3+ chelate spin labels, about 6nm apart, in a homodimer of the protein ERp29. While high-field DEER measurements on systems with such mixed labels can be highly attractive in terms of sensitivity and the potential to access long distances, a major difficulty arises...
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