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Overproduction of the short-chain dehydrogenase/reductase PTR1 confers resistance to the dihydrofolate reductase inhibitor methotrexate in the protozoan parasiteLeishmania.Genetic analysis has previously implicated PTR1 in pterin and folate metabolism. PTR1 was purified from a fusion protein expressed inEscherichia coli.Purified PTR1 exhibits NADPH-dependent biopterin, dihydrobiopterin, folate, and...
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