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The phthalate dioxygenase system consists of the dioxygenase, PDO, which contains a Rieske [2Fe–2S] center and a Fe(II)-mononuclear center, and the reductase, PDR. Involvement of the distal end of the 105–125 loop of PDO in its interaction with PDR was tested by substituting charged residues in the loop with alanines and by replacing the conserved tryptophan-94. Compared to wild-type PDO, all variants...
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