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Genistein, a phytoestrogen, was subcutaneously (s.c.) injected to pregnant Sprague-Dawley CD rats on gestational days 16-20 at either 25mg (Group 1) or 5mg/day (Group 2). Female offspring of mothers not exposed to genistein during pregnancy received 12.5mg genistein s.c. at neonatal days 15 and 18 (Group 3), or received vehicle only (Group 4). At 35 days of age, 4-9 female offspring from each group...
Intrarectal infusion of a 0.15 ml solution containing 1.5 mg or 0.5 mg of N-methyl-N-nitrosourea (MNU) was given to female shrews from 6 weeks of age. Fifteen shrews were given 16 doses of 1.5 mg MNU administered biweekly (group 1), 15 shrews were given 24 doses of 0.5 mg MNU weekly (group 2), and four untreated shrews served as controls (group 3). Moribund shrews were killed during the course of...
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