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The complex sulfation motifs of heparan sulfate glycosaminoglycans (HS GAGs) play critical roles in many important biological processes. However, an understanding of their specific functions has been hampered by an inability to synthesize large numbers of diverse, yet defined, HS structures. Herein, we describe a new approach to access the four core disaccharides required for HS/heparin oligosaccharide...
Kleinste Mengen von Kohlenhydraten (≈1 zmol) können durch eine Echtzeitmethode basierend auf der Konjugation der Kohlenhydrate mit DNA‐Markern quantitativ nachgewiesen werden (siehe Bild). Die vorgestellte Methode (Glyco‐qPCR) bietet einen ultraempfindlichen Nachweis von Kohlenhydraten, der in der Glycobiologie sowie der Kohlenhydrat‐basierten Wirkstoffforschung Anwendung finden kann.
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