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Vanillic acid is an abundant renewable chemical useful for the biocatalytic synthesis of aromatics. The bacterium, Nocardia sp. NRRL 5646 efficiently catalyzes the decarboxylation of vanillic acid to the phenolic product, guaiacol. For purification and characterization of vanillic acid decarboxylase (VAD), a simple, rapid enzyme assay was devised to couple the vanillic acid decarboxylase reaction...
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