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This study describes an analysis of different treatments that influence the relative content and the midpoint potential of HP Cyt b559 in PS II membrane fragments from higher plants. Two basically different types of irreversible modification effects are distinguished: the HP form of Cyt b559 is either predominantly affected when the heme group is oxidized (“O-type” effects) or when it is reduced (“R-type”...
“Reduced minus oxidized” difference extinction coefficients Δɛ in the α-bands of Cyt b559 and Cyt c550 were determined by using functionally and structurally well-characterized PS II core complexes from the thermophilic cyanobacterium Thermosynechococcus elongatus. Values of 25.1±1.0 mM −1 cm −1 and 27.0±1.0 mM −1 cm −1 were obtained for Cyt b559 and Cyt c550, respectively...
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