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A new method for stereospecific assignment of prochiral methyl groups in proteins is presented in which protein samples are produced using U-[13C]glucose and subsaturating amounts of 2-[13C]methyl-acetolactate. The resulting non-uniform labeling pattern allows proR and proS methyl groups to be easily distinguished by their different phases in a constant-time two-dimensional 1H-13C correlation spectra...
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