Eric J. Hustedt

article

Long Distance Measurements up to 160 Å in the GroEL Tetradecamer Using Q‐Band DEER EPR Spectroscopy

Thomas Schmidt, Marielle A. Wälti, James L. Baber, Eric J. Hustedt, more

Angewandte Chemie > 128 > 51 > 16137 - 16141

Current distance measurements between spin‐labels on multimeric protonated proteins using double electron–electron resonance (DEER) EPR spectroscopy are generally limited to the 15–60 Å range. Here we show how DEER experiments can be extended to dipolar evolution times of ca. 80 μs, permitting distances up to 170 Å to be accessed in multimeric proteins. The method relies on sparse spin‐labeling, supplemented...

article

Long Distance Measurements up to 160 Å in the GroEL Tetradecamer Using Q‐Band DEER EPR Spectroscopy

Thomas Schmidt, Marielle A. Wälti, James L. Baber, Eric J. Hustedt, more

Angewandte Chemie International Edition > 55 > 51 > 15905 - 15909

Current distance measurements between spin‐labels on multimeric protonated proteins using double electron–electron resonance (DEER) EPR spectroscopy are generally limited to the 15–60 Å range. Here we show how DEER experiments can be extended to dipolar evolution times of ca. 80 μs, permitting distances up to 170 Å to be accessed in multimeric proteins. The method relies on sparse spin‐labeling, supplemented...

chapter

Saturation Transfer EPR

Albert H. Beth, Eric J. Hustedt

Biological Magnetic Resonance > Biomedical EPR, Part B: Methodology, Instrumentation, and Dynamics > Motion, Proteins, and Membranes > 369-407

Saturation transfer EPR (ST-EPR) has been utilized to characterize the very slow rotational diffusion of a wide range of membrane proteins over the past three decades. Development of computational algorithms for analyzing the ST-EPR data from membrane proteins undergoinguniaxial rotational diffusion and inclusion of these algorithms into global nonlinear least squares fitting routines has permitted...

article

CW dipolar broadening EPR spectroscopy and mechanically aligned bilayers used to measure distance and relative orientation between two TOAC spin labels on an antimicrobial peptide

Indra D. Sahu, Eric J. Hustedt, Harishchandra Ghimire, Johnson J. Inbaraj, more

Journal of Magnetic Resonance > 2014 > 249 > C > 72-79

An EPR membrane alignment technique was applied to measure distance and relative orientations between two spin labels on a protein oriented along the surface of the membrane. Previously we demonstrated an EPR membrane alignment technique for measuring distances and relative orientations between two spin labels using a dual TOAC-labeled integral transmembrane peptide (M2δ segment of Acetylcholine receptor)...

article

Inter-spin distance determination using L-band (1–2GHz) non-adiabatic rapid sweep electron paramagnetic resonance (NARS EPR)

Aaron W. Kittell, Eric J. Hustedt, James S. Hyde

Journal of Magnetic Resonance > 2012 > 221 > Complete > 51-56

Site-directed spin-labeling electron paramagnetic resonance (SDSL EPR) provides insight into the local structure and motion of a spin probe strategically attached to a molecule. When a second spin is introduced to the system, macromolecular information can be obtained through measurement of inter-spin distances either by continuous wave (CW) or pulsed electron double resonance (ELDOR) techniques....

article

The global analysis of DEER data

Suzanne Brandon, Albert H. Beth, Eric J. Hustedt

Journal of Magnetic Resonance > 2012 > 218 > Complete > 93-104

Double Electron–Electron Resonance (DEER) has emerged as a powerful technique for measuring long range distances and distance distributions between paramagnetic centers in biomolecules. This information can then be used to characterize functionally relevant structural and dynamic properties of biological molecules and their macromolecular assemblies. Approaches have been developed for analyzing experimental...

article

Structure of the Intracellular Ca2+-Binding Domain of NCX1.1 by Site Directed Spin Labeling

Neena Dixit, Sunghoon Kim, Gage Matthews, Eric J. Hustedt, more

Biophysical Journal > 2011 > 100 > 3 Supplement 1 > 146a

article

The Global Analysis of DEER Data

Eric J. Hustedt

Biophysical Journal > 2011 > 100 > 3 Supplement 1 > 143a

article

Site-Directed Spin-Labeling Studies: Structure of the AnkD34-Cdb3 Complex

Sunghoon Kim, Suzanne Brandon, Jarrod A. Smith, Sarah J. Edwards, more

Biophysical Journal > 2011 > 100 > 3 Supplement 1 > 145a

article

Structure and Dynamics of the Calcium Binding Domains of the Na/Ca Exchanger (NCX1.1) Determined by Site Directed Spin Labeling

Mrinalini Dixit, Sunghoon Kim, Eric J. Hustedt, Charles E. Cobb, more

Biophysical Journal > 2010 > 98 > 3 Supplement 1 > 399a-400a

article

Structure of the CDB3 - ankD34 Complex from Site - Directed Spin - Labeling Studies

Sunghoon Kim, Eric J. Hustedt, Suzanne Brandon, Charles E. Cobb, more

Biophysical Journal > 2010 > 98 > 3 Supplement 1 > 400a

article

Conformational Equilibria of Bulged Sites in Duplex DNA Studied by EPR Spectroscopy ^†

Alyssa L. Smith, Pavol Cekan, Greg P. Brewood, Tamara M. Okonogi, more

The Journal of Physical Chemistry B > 2009 > 113 > 9 > 2664 - 2675

article

Conformational Equilibria Of Bulged Sites In Duplex Dna Studied By EPR Spectroscopy And Differential Scanning Calorimetry

Bruce Robinson, Alyssa L. Smith, Pavol Chekan, Greg Brewood, more

Biophysical Journal > 2009 > 96 > 3 Supplement 1 > 346a

article

Structure of the cdb3-ankD34 Complex from Site Directed Spin Labeling Studies

Sunghoon Kim, Eric J. Hustedt, Suzanne Brandon, Charles E. Cobb, more

Biophysical Journal > 2009 > 96 > 3 Supplement 1 > 309a-310a

article

Calculation of the EPR Spectrum of a Small Nitroxide from Molecular Dynamics Simulations

Kelli N. Kazmier, Terry P. Lybrand, Eric J. Hustedt

Biophysical Journal > 2009 > 96 > 3 Supplement 1 > 310a-311a

article

Simulation of Nitroxide Electron Paramagnetic Resonance Spectra from Brownian Trajectories and Molecular Dynamics Simulations

Susan C. DeSensi, David P. Rangel, Albert H. Beth, Terry P. Lybrand, more

Biophysical Journal > 2008 > 94 > 10 > 3798-3809

A simulated continuous wave electron paramagnetic resonance spectrum of a nitroxide spin label can be obtained from the Fourier transform of a free induction decay. It has been previously shown that the free induction decay can be calculated by solving the time-dependent stochastic Liouville equation for a set of Brownian trajectories defining the rotational dynamics of the label. In this work, a...

article

Structure of the Cytoplasmic Domain of Erythrocyte Band 3 Hereditary Spherocytosis Variant P327R: Band 3 Tuscaloosa ^†

Zheng Zhou, Susan C. DeSensi, Richard A. Stein, Suzanne Brandon, more

Biochemistry > 2007 > 46 > 36 > 10248 - 10257

article

Dipolar Coupling between Nitroxide Spin Labels: The Development and Application of a Tether-in-a-Cone Model

Eric J. Hustedt, Richard A. Stein, Latsavongsakda Sethaphong, Suzanne Brandon, more

Biophysical Journal > 2006 > 90 > 1 > 340-356

A tether-in-a-cone model is developed for the simulation of electron paramagnetic resonance spectra of dipolar coupled nitroxide spin labels attached to tethers statically disordered within cones of variable halfwidth. In this model, the nitroxides adopt a range of interprobe distances and orientations. The aim is to develop tools for determining both the distance distribution and the relative orientation...

article

Solution Structure of the Cytoplasmic Domain of Erythrocyte Membrane Band 3 Determined by Site-Directed Spin Labeling ^†

Zheng Zhou, Susan C. DeSensi, Richard A. Stein, Suzanne Brandon, more

Biochemistry > 2005 > 44 > 46 > 15115 - 15128

article

Formulation of Zeeman modulation as a signal filter

Robert D. Nielsen, Eric J. Hustedt, Albert H. Beth, Bruce H. Robinson

Journal of Magnetic Resonance > 2004 > 170 > 2 > 345-371

The Bloch equation containing a Zeeman modulation field is solved analytically by treating the Zeeman modulation frequency as a perturbation. The absorption and dispersion signals at both 0° and 90° modulation phase are obtained. The solutions are valid to first order in the modulation frequency, but are otherwise valid for any value of modulation amplitude or microwave amplitude. A first order treatment...

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Search results for: Eric J. Hustedt

Long Distance Measurements up to 160 Å in the GroEL Tetradecamer Using Q‐Band DEER EPR Spectroscopy

Long Distance Measurements up to 160 Å in the GroEL Tetradecamer Using Q‐Band DEER EPR Spectroscopy

Saturation Transfer EPR

CW dipolar broadening EPR spectroscopy and mechanically aligned bilayers used to measure distance and relative orientation between two TOAC spin labels on an antimicrobial peptide

Inter-spin distance determination using L-band (1–2GHz) non-adiabatic rapid sweep electron paramagnetic resonance (NARS EPR)

The global analysis of DEER data

Structure of the Intracellular Ca2+-Binding Domain of NCX1.1 by Site Directed Spin Labeling

The Global Analysis of DEER Data

Site-Directed Spin-Labeling Studies: Structure of the AnkD34-Cdb3 Complex

Structure and Dynamics of the Calcium Binding Domains of the Na/Ca Exchanger (NCX1.1) Determined by Site Directed Spin Labeling

Structure of the CDB3 - ankD34 Complex from Site - Directed Spin - Labeling Studies

Conformational Equilibria of Bulged Sites in Duplex DNA Studied by EPR Spectroscopy ^†

Conformational Equilibria Of Bulged Sites In Duplex Dna Studied By EPR Spectroscopy And Differential Scanning Calorimetry

Structure of the cdb3-ankD34 Complex from Site Directed Spin Labeling Studies

Calculation of the EPR Spectrum of a Small Nitroxide from Molecular Dynamics Simulations

Simulation of Nitroxide Electron Paramagnetic Resonance Spectra from Brownian Trajectories and Molecular Dynamics Simulations

Structure of the Cytoplasmic Domain of Erythrocyte Band 3 Hereditary Spherocytosis Variant P327R: Band 3 Tuscaloosa ^†

Dipolar Coupling between Nitroxide Spin Labels: The Development and Application of a Tether-in-a-Cone Model

Solution Structure of the Cytoplasmic Domain of Erythrocyte Membrane Band 3 Determined by Site-Directed Spin Labeling ^†

Formulation of Zeeman modulation as a signal filter

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