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Phosphate anions accelerate the oxidative folding of reduced bovine pancreatic ribonuclease A with dithiothreitol at several temperatures and ionic strengths. The addition of 400 mM phosphate at pH 8.1 increased the regeneration rate of native protein 2.5-fold at 15 o C, 3.5-fold at 25 o C, and 20-fold at 37 o C, compared to the rate in the absence of phosphate. In addition,...
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