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Purpose. To determine the effect of tether length on bone mineral affinity of fetuin-bisphosphonate conjugates. Methods. 1-Amino-1,1-diphosphonate methane (aminoBP) was conjugated onto the lysine residues of fetuin by using five different cross-linkers that varied in length. Both the conjugation efficiency (i.e., the number of aminoBPs per protein) as well as molecular dynamics modeling of the...
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