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Phosphorus (P) is one of the least-available nutrients in soils; improvement in plant P acquisition will significantly impact agriculture and the environment. A purple acid phosphatase (MtPAP1) cDNA was isolated from the model legume Medicago truncatula. During growth in high-phosphate conditions, MtPAP1 transcripts were highest in leaves, while under low-phosphate conditions, MtPAP1 transcript levels...
A full-length cDNA encoding an extracellular form of phytase was isolated from the model legume Medicago truncatula. The phytase cDNA (MtPHY1) has an open reading frame of 1,632 bp predicted to encode 543 amino acids including an N-terminal signal peptide of 27 amino acids. The MtPHY1 gene is 5,151 bp in length, containing 7 exons and 6 introns. MtPHY1 transcripts were detected in leaves and roots...
A novel, constitutively expressed gene, designated MtHP, was isolated from the model legume species Medicago truncatula. Sequence analysis indicates that MtHP most likely belongs to the PR10 multi-gene family. The MtHP promoter was fused to a β-glucuronidase gene to characterize its expression in different plant species. Transient assay by microprojectile bombardment and hairy root transformation...
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