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With a unique inheritance pattern compared to autosomal short tandem repeats (A‐STRs), X chromosomal STRs (X‐STRs) have special usage in forensic relationship testing. In this study, we designed a multiplex amplification system (named TYPER‐X19 multiplex assay) consisting of 18 STR loci spreading from 7.837 to 149.460 Mb on the X chromosomes (DXS9895, DXS8378, DXS9902, DXS6810, DXS7132, DXS10079,...
Massively parallel sequencing (MPS) technologies, also termed as next‐generation sequencing (NGS), are becoming increasingly popular in study of short tandem repeats (STR). However, current library preparation methods are usually based on ligation or two‐round PCR that requires more steps, making it time‐consuming (about 2 days), laborious and expensive. In this study, a 16‐plex STR typing system...
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