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In this work, a label-free and Raman dyes-free surface enhanced Raman spectroscopy for DNA detection based on the in situ DNA-metallization was developed and studied. A glass slide was employed to immobilize a peptide nucleic acid (PNA) as a recognition probe and to perform the detection procedure. Upon hybridization of the target DNA with the PNA probe, the DNA skeleton could adsorb positively charged...
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