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Here, we report a novel method consisting of capillary electrophoretic separation followed by two‐color LIF detection with postcolumn derivatization. The method can be used to discriminate glycoproteins in a protein mixture containing both glycosylated and unglycosylated proteins. The detector permitted simultaneous measurements of two electropherograms obtained by 450 nm (diode laser) and 532 nm...
Previously, we have demonstrated postcolumn derivatization of proteins separated by capillary sieving electrophoresis (CSE), in which naphthalene‐2,3‐dicarbaldehyde was employed as a fluorogenic labeling reagent. Standard proteins separated by CSE were reacted with naphthalene‐2,3‐dicarbaldehyde in the presence of 2‐mercaptoethanol (2‐ME) which plays a role of a reducing agent in the derivatization...
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