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In this paper, we report the dual function of 12-hydroxy octadecanoic acid (HOA)-modified barium titanate nanoparticles (BaTiO 3 NPs) as the matrix for phospholipids (PLs) and as hydrophobic affinity probes for liquid–liquid microextraction (LLME) of hydrophobic proteins in Escherichia coli prior to their identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry...
We propose the benefits of preincubation during nanoparticle‐assisted bacterial analysis, where the bacteria are grown along with the nanoparticles. We were able to obtain a two to ten fold enhancement of bacterial signals in 3 h compared to the generally used methodology followed in previous literature. The previous literature method required a long time (18 h) to obtain such an enhancement. We probe...
We describe the use of magnesium oxide nanoparticles (MgO-NPs) as a new matrix for the analysis of oligosaccharides (maltoheptaose, palatinose, D-panose, N-acetyllactosamine and β-gentiobiose) by surface-assisted laser desorption-ionization time-of-flight mass spectrometry (SALDI-MS). The use of MgO-NPs results in mass spectra without background noise and no fragmented ions in the low-mass region...
CdS quantum dots (∼5nm) are used as multifunctional nanoprobes as an effective matrix for large proteins, peptides and as affinity probes for the enrichment of tryptic digest proteins (lysozyme, myoglobin and cytochrome c) in laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS). The use of CdS quantum dots (CdS QDs) as the matrix allows acquisition of high resolution LDI mass...
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