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The effects of Diethyl aminoethyl hexanoate (DAH) on one-step micro-propagation culture with leave, petiole, and root explants were investigated. It was found that adding DAH into basic media increased the one-step culture rates from about less than 40% to more than 50%. For leaf explants, the optimum DAH concentration is 0.08 mg·L-1; petiole, 0.32 mg·L-1, and root explant,...
A strategy of repeatedly inducing axillary bud formation from bud explants was tested for micro propagation of purple coneflower (Echinacea purpurea L.) of different gene dosages. It was found that explants of lower gene dosage required lower concentrations of cytokinin (BA) and auxin (NAA) to achieve higher propagation rate of axillary buds, and with the most suitable BA and NAA concentrations tested,...
Newcastle disease (ND) is one of the most endangering diseases to poultry industry. Appropriate drug therapy is necessary. In present study, effects of Echinacea Purpurea (EP), Qingjie Compound (QC), and Echinacea Purpurea Compound formula (EPQC) on Newcastle disease virus (NDV) were investigated using chicken embryonic fibroblasts cells (CEF) in vitro and by NDV-infected chicken model in vivo. The...
An efficient in vitro propagation culture protocol was developed for one of the most important herb medicinal plants purple coneflower. Under the protocol, adventitious buds were first regenerated from leaf, petiole and root explants of aseptic seedlings on MS medium with 0.3 mg/l BA and 0.01 mg/l NAA, and then these adventitious buds were rooted and grown into plantlets of certain maturity on MS...
The main objective for this study was to evaluate the influence of gene dose on culture responses of adventitious buds regeneration in one of the most important medicinal plant purple coneflower. Explants of leaf, petiole and root with same gene composition but of various doses of one set, two sets and four sets of chromosomes were taken from in vitro cultured haploid (2n=1X=11), diploid (2n=2X=22)...
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