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Urea-extractable proteins have been purified from the cephalothoracic cuticle of mature Araneus diadematus spiders. Two-dimensional gel electrophoresis showed at least 12 major proteins, with pls between 4.5 and 8.5. Five proteins were purified and their primary structure determined, using a combination of mass spectrometry and Edman degradation. Based on the amino acid sequence the proteins can be...
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