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The genome of Corynebacterium glutamicum NCHU 87078 contains two putative thymidylate synthase genes, designated CgthyA and CgthyX. These two genes were expressed in Escherichia coli NovaBlue and the expressed His 6 -tagged enzymes were purified by nickel-chelate chromatography. The purified CgThyA had a specific activity of 414mU mg −1 protein, whereas thymidylate synthase activity...
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