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In this study, we demonstrated a graphene oxide (GO)-based immunobiosensor system for pathogen detection using fluorescence quenching effect between GO and gold nanoparticles. The fluorescent GO sheets was deposited on an amino-modified glass surface by electrostatic force, and the carboxylate functional group on the GO surface was used to covalently conjugate rotavirus-antibodies to be linked on...
Don′t FRET when I GO: Sensitive and selective rotavirus detection is achieved by using the photoluminescence of a graphene oxide (GO) array. The target cell was captured by the rotavirus‐specific antibody immobilized on the GO array, and the binding event was monitored by observing the fluorescence quenching that results from fluorescence resonance energy transfer (FRET) between GO and gold nanoparticles...
We present here a miniaturized lab-on-a-chip (LOC) system that integrates cell capture and preconcentration, polymerase chain reaction (PCR), and capillary electrophoresis (CE) for pathogen detection. For efficient cell capture on a chip, we used an optimized bifurcated microfluidic channel design and a pumping flutter scheme to isolate target cells, E. coli K12 and E. coli 0157, in a fluidized bed...
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