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An electrochemical hybridization biosensor was developed for the detection of short DNA fragments specific to the deadly waterborne pathogen Cryptosporidium. The sensor relies on the immobilization of a 38-mer oligonucleotide unique to the Cryptosporidium DNA onto the carbon-paste transducer, and employs a highly sensitive chronopotentiometric transduction mode for monitoring the hybridization event...
An electrochemical biosensor for the determination of short sequences from the Mycobacterium tuberculosis (MTB) DNA is described. The sensor relies on the modification of the carbon-paste transducer with 27- or 36-mer oligonucleotide probes and their hybridization to complementary strands from the MTB DNA direct repeat region. Chronopotentiometry is employed to transduce the hybridization event,...
DNA hybridization on carbon paste electrodes was followed by stripping potentiometric measurement of electroactive metal complexes associated with surface hybrids. The chronopotentiometric transduction mode offers high sensitivity and effective background compensation, and improves the sequence detection compared to analogous (cyclic- and square-wave) voltammetric measurements. Immobilization of...
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