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In the P450cam reaction cycle, the interactions between putidaredoxin (Pdx) and P450cam or NADH-putidaredoxin reductase (PdR) have been considered to be essential in the electron-transfer process. In this study, three mutant putidaredoxins were prepared to examine the hydrophobic and electrostatic interactions in the reaction complexes. Val-98, which is exposed to solvent and has been previously...
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