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A new method was developed to prepare monolithic immobilized pH gradient (M‐IPG) columns in UV‐transparent fused‐silica capillaries by the 5‐min photopolymerization of acrylamide and N,N′‐methylenebisacrylamide, followed by the 20‐min photografting of the focused ampholine‐derived glycidylmethacrylate monomer on the monolithic matrix, by which the preparation time was reduced, and the stability of...
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