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Lipid analogues carrying three nitrilotriacetic acid (tris‐NTA) head groups were developed for the selective targeting of His‐tagged proteins into liquid ordered (lo) or liquid disordered (ld) lipid phases. Strong partitioning into the lo phase of His‐tagged proteins bound to tris‐NTA conjugated to saturated alkyl chains (tris‐NTA DODA) was achieved, while tris‐NTA conjugated to an unsaturated alkyl...
Proteine in der Zelle verfolgen: Mithilfe einer Klick‐Reaktion wurde ein optimiertes Substrat hergestellt, um im Zytoplasma lebender Zellen Nanopartikel spezifisch und irreversibel an Proteine anzubinden, die an eine modifizierte Halogenalkan‐Dehalogenase fusioniert sind. So konnten Proteine in der äußeren Mitochondrienmembran über längere Zeit hinweg mit einer Auflösung von wenigen Nanometern verfolgt...
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