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We have developed a novel surface plasmon resonance (SPR) biosensor for heme detection that utilizes the reconstitution of the heme cofactor with apohemoglobin (apoHb), hemoglobin from which the heme has been removed, as the sensing mechanism. The binding is highly specific, efficient and generated very strong SPR signals. This is the first report that uses immobilization of the apoprotein in a hydrophilic...
Signal amplifiers: Superparamagnetic particle–antibody conjugates were used to detect a cancer biomarker in serum by surface plasmon resonance (SPR) at an unprecedented low level of 10 fg mL−1 (ca. 300 aM). Ultrahigh sensitivity is facilitated by enhanced mass and refractive index from aggregates of 1 μm magnetic particles on the SPR chip.
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