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Two polar silica bonded stationary phases were prepared by first functionalizing the silica surface with γ‐glycidoxypropyltrimethoxysilane, which was then reacted with TRIS to yield a polyhydroxy surface that also has secondary amine functionalities. This step produced the singly layered TRIS‐silica column, a cationic hydrophilic column. The TRIS‐silica surface was further coated with a layer of chondroitin...
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