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The role of nitric oxide (NO) as a regulatory diffusible molecule in the brain requires the evaluation of its concentration dynamics. In this work, we have developed microelectrodes suitable for real time electrochemical measurements of NO in vitro. Nafion and o-phenylenediamine were used to modify the surface of carbon fiber microelectrodes (8μm diameter; ≈100μm tip length). Coating with Nafion was...
A ceramic-based multisite microelectrode array to measure choline in vivo in brain tissues is described. The microelectrodes were linear to 200μM choline (R 2 =0.999+/-0.001) with a detection limit of approximately 0.4μM (S/N of 3) in both single microelectrode and self-referencing amperometric recording modes. The 90% rise time of the sensor was 1.4s, allowing for rapid measures of choline...
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