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In Echtzeit und ratiometrisch kann die Thrombin‐Aktivierung in vivo mithilfe aktivierbarer zellgängiger Peptide (RACPPs) verfolgt werden. RACPPs verbinden die 1) Ermittlung des Verhältnisses zweier Emissionen, 2) In‐vivo‐Bildgebung in Säugern im fernen Rot‐ bis Infrarotbereich und 3) spaltungsabhängige räumliche Lokalisierung. Das beste RACPP nutzt Norleucin(Nle)‐TPRSFL als Linker, was die Empfindlichkeit...
In real time: Thrombin activation in vivo can be imaged in real time with ratiometric activatable cell penetrating peptides (RACPPs). RACPPs are designed to combine 1) dual‐emission ratioing, 2) far red to infrared wavelengths for in vivo mammalian imaging, and 3) cleavage‐dependent spatial localization. The most advanced RACPP uses norleucine (Nle)‐TPRSFL as a linker that increases sensitivity to...
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