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A highly robust hydrophilic interaction liquid chromatography (HILIC) method that involves both fluorescence and mass spectrometric detection was developed for profiling and characterizing enzymatically released and 2‐aminobenzamide (2‐AB)‐derivatized mAb N‐glycans. Online HILIC/mass spectrometry (MS) with a quadrupole time‐of‐flight mass spectrometer provides accurate mass identifications of the...
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