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AimsThe study aims to develop a novel multistep chromatographic purification process for human enterovirus71 virus‐like particles (VLPs) produced from insect cells (Sf9) infected with recombinant baculovirus.
Methods and ResultsSf9 cells were maintained in the Wave Bioreactor system 20/50, and harvested when the viability decreased to 75% after infected with Bac‐P1‐3CD at the multiplicity of infection...
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