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The use of minimal gene cassettes (MCs), which are linear DNA fragments (promoter+open reading frame+terminator) lacking the vector backbone sequence, was compared to the traditional use of whole circular plasmids (CPs) for transformation of grapevine. Embryogenic cell suspensions of ‘Chardonnay’ (Vitis vinifera L.) were transformed via particle co-bombardment using two nonlinked genes in either MCs...
A reliable and efficient system for transformation and regeneration of Chardonnay (Vitis vinifera L.) plants via microprojectile bombardment was developed. Improvements over the previous biolistic transformation system included: (1) the use of gold particles for bombardment; (2) step-wise selection at 10 then 15mg/l kanamycin; and (3) embryo induction at 27C. Embryogenic cell cultures were either...
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