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An endogenous ligand of the 78 kDa gastrin-binding protein (GBP) has been purified from detergent extracts of porcine gastric mucosal membranes by ion exchange chromatography and preparative gel electrophoresis. The ligand bound to the GBP with high affinity (mean IC 50 value of 0.31+/-0.09 μg/ml, or 8 nM), as assessed by inhibition of cross-linking of iodinated gastrin 2, ...
A heat-stable wheat protein (WP) that is a good substrate for wheat embryo Ca 2+ -dependent protein kinase (CDPK) was purified from wheat embryo by a procedure involving batchwise anion exchange chromatography on DEAE-cellulose (DE52), passage through Phenyl-Sepharose CL-4B, heat and acid treatment and anion exchange HPLC on a DEAE-5PW column. WP is phosphorylated by CDPK to a stoichiometry...
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