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Background
Virus inactivation is a critical operation in therapeutic protein manufacturing. Low pH buffers are a widely used strategy to ensure robust enveloped virus clearance. However, the choice of model virus can give varying results in viral clearance studies. Pseudorabies virus (SuHV) or herpes simplex virus‐1 (HSV‐1) are frequently chosen as model viruses to demonstrate the inactivation for...
Due to the high variation in viral surface properties, a platform method for virus purification is still lacking. A potential alternative to the high‐cost conventional methods is aqueous two‐phase systems (ATPSs). However, optimizing virus purification in ATPS requires a large experimental design space, and the optimized systems are generally found to operate at high ATPS component concentrations...
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