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An iterative approach to optimising the accumulation of non-native long chain polyunsaturated fatty acids in transgenic plants was undertaken in Arabidopsis thaliana. The contribution of a number of different transgene enzyme activities was systematically determined, as was the contribution of endogenous fatty acid metabolism. Successive iterations were informed by lipidomic analysis of neutral, polar...
The nutritional importance of long chain polyunsaturated fatty acids (LC-PUFAs) is now well-established, with these lipids playing roles in human development and the prevention of various diseases. In particular, n-3 polyunsaturated fatty acids are protective against cardiovascular disease (CVD) and risks associated with metabolic syndrome. In view of the decline in marine fish stocks, which represent...
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