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Placental alkaline phosphatase is an inducible enzyme, expressed in HeLaS3 cells, which has been shown to possess protein phosphotyrosine phosphatase activity. Since phosphotyrosine levels are known to increase in actively dividing cells we sought an inverse correlation between PLAP activity and growth rate in HeLaS3 cells. We found that PLAP inducers, Na-butyrate, dexamethasone, bromodeoxyuridine...
When chick cardiac cells are maintained in serum-free NCTC-135 medium, the pH i dependence of the Na + / H + exchanger is much more acidic (pK = 7.0) than when a serum-free M199 medium is used (pK > 7.5). Phorbol esters activate the Na + /H + exchanger and produce a cell alkalinization in cells maintained in NCTC-135 medium by shifting the pH i dependence...
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