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Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethanefoam (PUF) and grew to the high density of 6.83107cells/cm3 PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/106 cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system...
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