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Here, we describe the first example of a cell-based myosin light chain phosphorylation assay in 96-well format that allows for the rapid screening of novel Rho-kinase inhibitors. We obtained IC 50 values for the prototypic Rho-kinase inhibitors Y-27632 (1.2±0.05μM) and Fasudil (3.7±1.2μM) that were similar to those previously published utilizing electrophoresis-based methodologies. H-1152P,...
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