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In several organisms used for recombinant protein production, integration of the expression cassette into the genome depends on site-specific recombination. In general, the yeast Kluyveromyces lactis shows low gene-targeting efficiency. In this work, two K. lactisku80− strains defective in the non-homologous end-joining pathway (NHEJ) were constructed using a split-marker strategy and tested as...
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