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MALDI-MS was used to confirm the cDNA derived amino acid sequence of HMW subunit 1Dx5 and an E. coli -expressed repetitive M r 58 000 peptide based on residues 102–643. Analysis of theM r 58 000 peptide and of tryptic peptides separated by RP-HPLC showed that the experimentally determinedM r are consistent, with the calculated values, within the experimental error. This indicates...
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